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Noise Terminator | Noise Terminator

Key Words: photobleaching, phototoxicity, fluorescence, signal-to-noise ratio, Filter block

Definition:A device that increase the fluorescence signal-to-noise ratio by helping to prevent any stray light in the filter block from reaching the detector (eyes, camera etc)

TECHNOLOGY:

Some of the shorter and longer wavelengths that are able to pass through the excitation filter in the filter block may not be deflected by the dichromatic mirror and pass through. A portion of these photons, which are a major source of noise in fluorescence microscopy, reflect from the interior of the filter block and are able to cross the barrier filter at oblique angles to reach the detector. The Noise Terminator is a device positioned at the rear of a filter block. Photons flowing through the rear of the optical block into the terminator tube are absorbed by neutral density material, thus escaping the microscope optical path. Any light that does not get absorbed by the neutral density material is reflected into the end of the terminator tube where it is finally dispersed.

APPLICATIONS:

The increase signal-to-noise ratio and, therefore, increased contrast achieved through the use of Noise Terminator technology has particular advantages in imaging dim fluorescence. It also gives users a greater opportunity for reducing the intensity of light required to provide sufficient excitation for imaging - a great advantage in reducing phototoxicity and photobleaching in live cell imaging applications.

MICROSCOPE CONFIGURATION:

Noise Terminator technology is incorporated in Nikon's Ti series inverted microscopes and Eclipse 90i and 80i upright microscopes.

RECOMMENDED SYSTEM:

For live cell imaging the Ti series inverted series of microscopes incorporating Noise Terminator technology provides great flexibility in accommodating a wide range of live cell imaging applications.

LINKS:

Interactive noise terminator tutorial: [microscopyu]

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