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Field-of-View | Field-of-View

Key Words: parfocal, digital imaging, Magnification, parcentred, virtual slide

Definition:The area of the specimen visible through the microscope eyepiece

TECHNOLOGY:

The field-of-view observed through the microscope objective is inversely proportional to magnification - i.e. the greater the magnification the smaller the field-of-view. Parfocal and parcentred objectives facilitate switching between magnifications (and fields-of-view) without significant loss of focus or without 'losing' the subject under high power. In some digital analysis and imaging systems it is possible to display an overview (large field-of-view image) at the same time as viewing a high magnification image. This allows users to easily locate the high power region being imaged on the whole specimen. Digital imaging also enables larger fields-of-view to be created by 'stitching' adjacent images together for viewing on a PC screen.

APPLICATIONS:

A range of fields-of view (magnifications) is an advantage in providing an overall understanding of the specimen. A low power objective (large field-of-view) such as 1X - 5X times is particularly useful in clinical pathology applications where it provides a comprehensive overview to enable identification of features of possible diagnostic significance for further high power imaging.

MICROSCOPE CONFIGURATION:

As the field-of-view is dictated by the magnification, a range of objectives may be important in providing an overall understanding of specimen features. The precise range used will depend on the application.

RECOMMENDED SYSTEM:

For clinical applications the Coolscope digital microscope system provides an all-digital solution where specimens are viewed directly on a PC screen. The entire area of a single glass slide can be viewed on screen. This can be maintained as a thumb nail reference image while examining selected areas under high magnification. Areas being imaged at higher powers are automatically tracked on the overview image using a crosshair marker to maintain an overall understanding of the geography of the specimen.

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