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Diascopic Illumination | Diascopic Illumination

Key Words: specimen characteristics, geometrical profile, topography, composition, morphology

Definition:Determining specimen characteristics, including geometrical profile, topography, composition and morphology of the specimen is important when choosing a suitable illumination strategy for optimum imaging; opacity of the specimen, being generally the most important characteristic

Diascopic Illumination

Translucent and transparent specimens produce the clearest and most vivid images when lit by transmitted illumination, be it brightfield, polarized, oblique or darkfield. Diascopic light transmitted at an oblique angle can often deliver greater contrast and to produce images of enhanced clarity.

However, it should also be noted that many objects reveal their characteristics best through a combination of both transmitted and reflected light

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